Isolation of avian influenza virus from backyard poultry population of tehsil Abbottabad, Khyber Pakhtunkhwa, Pakistan
DOI:
https://doi.org/10.55627/zoobotanica.002.01.0647Keywords:
Avian influenza virus, backyard chickens, golden, fayomi , aseelAbstract
The avian influenza virus is a highly contagious viral disease that predominantly impacts avian species, especially domestic birds like chickens, ducks, and turkeys. The study was conducted to isolate the avian influenza virus from domestic poultry breeds (Desi, Aseel, Fayoumi, and Golden) from backyard poultry population of the Tehsil Abbottabad from January to June 2023. A cross-sectional study was conducted in three rural and five urban areas of Tehsil Abbottabad Using sterile cotton swabs, 128 (tracheal and cloacal) swab samples were collected. The samples were labelled and stored in a brain-heart infusion medium, then transported to the National Reference Laboratory for Poultry Diseases in Islamabad using a thermostat shipment box. The samples were processed using a real-time reverse transcription polymerase chain reaction for avian influenza virus detection. This study concludes with a significant 9.38% prevalence percentage of avian influenza virus in Tehsil Abbottabad backyard poultry. The prevalence percentage of H9 was 83.33%, and that of H5 was 16.66%. The findings reveal the importance of implementing preventative measures to curb avian influenza outbreaks within the poultry population of the district. This study offers vital data for AIV research in 2023, guiding enhanced containment and prevention in backyard poultry to control avian influenza.
References
Alexander, D. J. (2003). Report on avian influenza in the Eastern Hemisphere during 1997–2002. Avian Diseases, 47(s3), 792-797.
Aslam, H. B., Alarcon, P., Yaqub, T., Iqbal, M., & Häsler, B. (2020). A value chain approach to characterize the chicken sub-sector in Pakistan. Frontiers in Veterinary Science, 7, 361.
Cao, Y., Liu, H., Liu, D., Liu, W., Luo, T., & Li, J. (2022). Hemagglutinin gene variation rate of H9N2 avian influenza virus by vaccine intervention in China. Viruses, 14(5), 1043.
Chan, J. F. W., To, K. K. W., Chen, H., & Yuen, K. Y. (2015). Cross-species transmission and emergence of novel viruses from birds. Current opinion in virology, 10, 63-69.
Chang, P., Sadeyen, J. R., Bhat, S., Daines, R., Hussain, A., Yilmaz, H., & Iqbal, M. (2023). Risk assessment of the newly emerged H7N9 avian influenza viruses. Emerging Microbes & Infections, 12(1), 2172965.
Channa, A. A., Tariq, M., Nizamani, Z. A., & Kalhoro, N. H. (2022). Prevalence of avian influenza h5, h7 and h9 viruses in commercial broilers at karachi, Pakistan. Journal Animal Health Production, 10(1), 29-34.
Chaudhry, M., Ahmad, M., Rashid, H. B., Sultan, B., Chaudhry, H. R., Riaz, A., & Shaheen, M. S. (2017). Prospective study of avian influenza H9 infection in commercial poultry farms of Punjab Province and Islamabad Capital Territory, Pakistan. Tropical Animal Health and Production, 49, 213-220.
Chaudhry, M., Webby, R., Swayne, D., Rashid, H. B., Dbeauchamp, J., Killmaster, L., & Hasni, M. S. (2020). Avian influenza at animal‐human interface: One‐health challenge in live poultry retail stalls of Chakwal, Pakistan. Influenza and other Respiratory Viruses, 14(3), 257-265.
Guo, F., Li, Y., Yu, S., Liu, L., Luo, T., Pu, Z., ... & Shen, Y. (2019). Adaptive evolution of human isolated H5Nx avian influenza a virus. Frontiers in Microbiology, 10, 1328.
Kargarfard, F., Sami, A., Mohammadi-Dehcheshmeh, M., & Ebrahimie, E. (2016). Novel approach for identification of influenza virus host range and zoonotic transmissible sequences by determination of host-related associative positions in viral genome segments. BMC genomics, 17, 1-10.
Khalid, N. (2020). Prevalence of Avian Influenza Virus in Poultry Population of District Mansehra. EC Veterinary Science, 5, 15-20.
Khan, M., Maqsood, R., Rashid, H. B., Gill, S. S., Noman, M., Akram, R., ... & Chaudhry, M. (2022). Seroprevalence of Avian Influenza H9N2 Virus in Backyard Poultry of District Zhob Pakistan. Pakistan Journal of Zoology 1-6.
Lefkowitz, E. J., Dempsey, D. M., Hendrickson, R. C., Orton, R. J., Siddell, S. G., & Smith, D. B. (2018). Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV). Nucleic Acids Research, 46(D1), D708-D717.
Liu, W. J., Wu, Y., Bi, Y., Shi, W., Wang, D., Shi, Y., & Gao, G. F. (2022). Emerging HxNy influenza A viruses. Cold Spring Harbor Perspectives in Medicine, 12(2), a038406.
Monika, M. B., Padmanabha, B., & Channaveerappa, H. (2018). Ninhydrin Positive Eggshell Surface Proteins and Water-Soluble Proteins of Six Domesticated Birds can Become Possible Tools to Assess Taxonomic as well Genetic Diversity. International Journal of Zoological Investigations, 4 (1), 88- 96.
Navid, M. T., Awais, M. M., Anwar, M. I., & Akhtar, M. (2022). Seroprevalence of Avian Influenza Viruses in Asymptomatic Backyard Poultry Birds in District Multan, Pakistan. Pakistan Journal of Zoology 1-7.
Rehman, S., Khan, M. I., Rantam, F. A., Effendi, M. H., Shehzad, A., & Tariq, A. (2021). Seroprevalence and associated risk factors of avian influenza virus subtype H9N2 in backyard poultry of Peshawar Pakistan. Journal of the Indonesian Tropical Animal Agriculture 46(3): 2087-8273.
Resa-Infante, P., Jorba, N., Coloma, R., & Ortin, J. (2011). The influenza virus RNA synthesis machine: advances in its structure and function. RNA Biology, 8(2), 207-215.
Sarwar, M., Muhammad, K., Rabbani, M., Younus, M., Sarwar, N., Ali, M. A., & Ahad, A. (2013). Prevalence of Avian Influenza Viruses in live bird markets of Lahore. The Journal of Animal & Plant Sciences, 23(2): 388-392
Spackman, E., Senne, D. A., Myers, T. J., Bulaga, L. L., Garber, L. P., Perdue, M. L., ... & Suarez, D. L. (2002). Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. Journal of Clinical Microbiology, 40(9), 3256-3260.
Suarez, D. L., & Schultz-Cherry, S. (2000). Immunology of avian influenza virus: a review. Developmental & Comparative Immunology, 24(2-3), 269-283.
Swayne, D. E., Hill, R. E., & Clifford, J. (2017). Safe application of regionalization for trade in poultry and poultry products during highly pathogenic avian influenza outbreaks in the USA. Avian Pathology, 46(2), 125-130.
Swayne, D. E., Spackman, E., & Pantin-Jackwood, M. (2014). Success factors for avian influenza vaccine use in poultry and potential impact at the wild bird–agricultural interface. EcoHealth, 11, 94-108.
Taubenberger, J. K., & Morens, D. M. (2006). 1918 Influenza: the mother of all pandemics. Revista Biomedica, 17(1), 69-79.
Van de Sandt, C. E., Kreijtz, J. H., & Rimmelzwaan, G. F. (2012). Evasion of influenza A viruses from innate and adaptive immune responses. Viruses, 4(9), 1438-1476.
Wahlgren, J. (2011). Influenza A viruses: an ecology review. Infection Ecology & Epidemiology, 1(1), 6004.
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